HOMER1 Monoclonal antibody

HOMER1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig, Chicken

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3B1B11

Cat no : 68597-1-Ig

Synonyms

HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1, HOMER1, HOMER1A, HOMER1B, HOMER1C, SYN47, Ves 1

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Validation Data Gallery

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  WB analysis using 68597-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 68597-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 68597-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68597-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of SH-SY5Y using 68597-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using HOMER1 antibody (68597-1-Ig, Clone: 3B1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: pig brain tissue, LNCaP cells, Calu-3 cells, HEK-293 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue, MCF-10A cells, MOLT-4 cells, rat brain tisue, chicken brain tissue
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68597-1-Ig targets HOMER1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig, Chicken samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HOMER1 fusion protein Ag21302
• Full Name: homer homolog 1 (Drosophila)
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40-46 kDa
• GenBank Accession Number: BC015502
• Gene Symbol: HOMER1
• Gene ID (NCBI): 9456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.