HO-1 Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

HO-1 Uni-rAb^TM Recombinant Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2M6

Cat no : 81281-1-PBS

Synonyms

HMOX1, HO-1/HMOX1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1

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Validation Data Gallery

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  WB analysis using 81281-1-RR (same clone as 81281-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81281-1-RR (HO-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 81281-1-RR (same clone as 81281-1-PBS)

  WB result of HO-1 antibody (81281-1-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HO-1 transfected HeLa cells. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 81281-1-RR (same clone as 81281-1-PBS)

  IP result of anti-HO-1 (IP:81281-1-RR, 4ug; Detection:81281-1-RR 1:8000) with HeLa cells lysate 1120 ug. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81281-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81281-1-RR against Human HO-1 were performed. The affinity constant is below 1 pM.

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Product Information

81281-1-PBS targets HO-1 in WB, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HO-1 fusion protein Ag1190
• Full Name: heme oxygenase (decycling) 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC001491
• Gene Symbol: HO-1
• Gene ID (NCBI): 3162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663).