HNRPDL Polyclonal antibody

HNRPDL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10660-2-AP

Synonyms

hnRNP D-like, hnRNP DL, hnRNP D like, HNRNP, Heterogeneous nuclear ribonucleoprotein D-like

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Validation Data Gallery

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  WB analysis using 10660-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10660-2-AP (HNRPDL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 10660-2-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10660-2-AP (HNRPDL antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells, K-562 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

10660-2-AP targets HNRPDL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HNRPDL fusion protein Ag1046
• Full Name: heterogeneous nuclear ribonucleoprotein D-like
• Calculated Molecular Weight: 46 aa, 4 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC007392
• Gene Symbol: HNRPDL
• Gene ID (NCBI): 9987
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Cell Biochem Funct; Overexpression of JKTBP1 induces androgen-independent LNCaP cell proliferation through activation of epidermal growth factor-receptor (EGF-R).; Authors - Yong-Yang Wu