HNRNPR Polyclonal antibody, PBS Only

HNRNPR Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29980-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 29980-1-AP (same clone as 29980-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29980-1-AP (HNRNPR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29980-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 29980-1-AP (same clone as 29980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29980-1-AP (HNRNPR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29980-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 29980-1-AP (same clone as 29980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29980-1-AP (HNRNPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29980-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 29980-1-AP (same clone as 29980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29980-1-AP (HNRNPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29980-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 29980-1-AP (same clone as 29980-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HNRNPR antibody (29980-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29980-1-PBS in a different storage buffer formulation.

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Product Information

29980-1-PBS targets HNRNPR in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HNRNPR fusion protein Ag30339
• Full Name: heterogeneous nuclear ribonucleoprotein R
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 65-82 kDa
• GenBank Accession Number: BC001449
• Gene Symbol: HNRNPR
• Gene ID (NCBI): 10236
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497)