HNRNPL Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

HNRNPL Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18354-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 18354-1-AP (same clone as 18354-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18354-1-AP (HNRNPL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 18354-1-AP (same clone as 18354-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18354-1-AP (HNRNPL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IP experiment of BxPC-3 using 18354-1-AP (same clone as 18354-1-PBS)

  IP result of anti-HNRNPL (IP:18354-1-AP, 4ug; Detection:18354-1-AP 1:4000) with BxPC-3 cells lysate 2080 ug. This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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Product Information

18354-1-PBS targets HNRNPL in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HNRNPL fusion protein Ag13211
• Full Name: heterogeneous nuclear ribonucleoprotein L
• Calculated Molecular Weight: 572 aa, 62 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC069184
• Gene Symbol: HNRNPL
• Gene ID (NCBI): 3191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. It is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. The two isoforms of hnRNP L are generated by alternative splicing. The human hnRNPL gene encodes a protein that is localized in the nucleoplasm and cytoplasm. This antibody may recognize both isoforms of the hnRNP L protein.