HNRNPK Monoclonal antibody, PBS Only

HNRNPK Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A10C8

Cat no : 67708-1-PBS

Synonyms

CSBP, FLJ41122, hnRNP K, HNRNPK, HNRPK, TUNP

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Validation Data Gallery

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  WB analysis using 67708-1-Ig (same clone as 67708-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67708-1-Ig (HNRNPK antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67708-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67708-1-Ig (same clone as 67708-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67708-1-Ig (HNRNPK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67708-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67708-1-Ig (same clone as 67708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67708-1-Ig (HNRNPK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67708-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67708-1-Ig (same clone as 67708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67708-1-Ig (HNRNPK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67708-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67708-1-Ig (same clone as 67708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67708-1-Ig (HNRNPK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67708-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67708-1-Ig (same clone as 67708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67708-1-Ig (HNRNPK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67708-1-PBS in a different storage buffer formulation.

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Product Information

67708-1-PBS targets HNRNPK in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: HNRNPK fusion protein Ag30093
• Full Name: heterogeneous nuclear ribonucleoprotein K
• Calculated Molecular Weight: 463 aa, 51 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC014980
• Gene Symbol: HNRNPK
• Gene ID (NCBI): 3190
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

HNRNPK belongs to the heterogeneous nuclear ribonucleoprotein family of 20 proteins that participate in a wide range of key cellular functions involving many of the pathways implicated, disrupted or dysregulated in tumor development and progression. It is also a conserved pre-mRNA-binding protein that is involved in multiple processes of gene expression, including chromatin remodeling, transcription, and mRNA splicing, translation, and stability