HNRNPH1 Monoclonal antibody

HNRNPH1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1A6C2

Cat no : 67375-1-Ig

Synonyms

DKFZp686A15170, hnRNP H, hnRNPH, HNRNPH1, HNRPH, HNRPH1

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Validation Data Gallery

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  WB analysis using 67375-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67375-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67375-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67375-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67375-1-Ig (HNRNPH1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, U2OS cells, HSC-T6 cells, 4T1 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67375-1-Ig targets HNRNPH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HNRNPH1 fusion protein Ag6603
• Full Name: heterogeneous nuclear ribonucleoprotein H1 (H)
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49-50 kDa
• GenBank Accession Number: BC001348
• Gene Symbol: HNRNPH1
• Gene ID (NCBI): 3187
• RRID: AB_2882623
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].

Publications

Species	Application	Title
human	WB	Front Oncol; MS0621, a novel small-molecule modulator of Ewing sarcoma chromatin accessibility, interacts with an RNA-associated macromolecular complex and influences RNA splicing; Authors - Tamara Vital
human	WB	J Cell Physiol; MACC1 enhances an oncogenic RNA splicing of IRAK1 through interacting with HNRNPH1 in lung adenocarcinoma; Authors - Shiqing Wang