HNRNPC Recombinant antibody

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  WB analysis using 82798-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82798-3-RR (HNRNPC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 82798-3-RR

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82798-3-RR (HNRNPC antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 82798-3-RR

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82798-3-RR (HNRNPC antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 82798-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82798-3-RR (HNRNPC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 82798-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82798-3-RR (HNRNPC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 82798-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPC antibody (82798-3-RR, Clone: 4F16 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). CoraLite® Plus 488-Phalloidin (green).

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  Affinity and Kinetic Characterization of 82798-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82798-3-RR against Human HNRNPC were performed. The affinity constant is below 1 pM.

HNRNPC Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 82798-3-RR

Clone No.4F16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HNRPC, hnRNP C1/C2, HNRNP, C2, C1

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells
• Positive IHC detected in: human stomach tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82798-3-RR targets HNRNPC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HNRNPC fusion protein Ag2356
• Full Name: heterogeneous nuclear ribonucleoprotein C (C1/C2)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 36-40 kDa
• GenBank Accession Number: BC003394
• Gene Symbol: HNRNPC
• Gene ID (NCBI): 3183
• RRID: AB_3086542
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07910
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.