HNRNPC Monoclonal antibody

HNRNPC Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 68447-1-Ig
Clone No.2G8G4

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse , rat

Applications

WB, IF/ICC, IP, ELISA

C1, C2, HNRNP, hnRNP C1/C2, HNRNPC, HNRPC, SNRPC

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inU2OS cells, A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68447-1-Ig targets HNRNPC in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Reactivity Human, mouse , rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HNRNPC fusion protein Ag2356 Predict reactive species
Full Name heterogeneous nuclear ribonucleoprotein C (C1/C2)
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 36-40 kDa
GenBank Accession NumberBC003394
Gene Symbol HNRNPC
Gene ID (NCBI) 3183
RRIDAB_3085159
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP07910
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNRNPC, also named as Heterogeneous nuclear ribonucleoproteins C1/C2, is a 306 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and belongs to the RRM HNRPC family. HNRNPC localizes in the nucleus. It binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. HNRNPC may play a role in the early steps of spliceosome assembly and pre-mRNA splicing. HNRNPC can act as a tetramer and is involved in the assembly of 40S hnRNP particles and plays a role with CSDE1 in internal initiation of translation during mitosis.

Protocols

Product Specific Protocols
WB protocol for HNRNPC antibody 68447-1-IgDownload protocol
IF protocol for HNRNPC antibody 68447-1-IgDownload protocol
IP protocol for HNRNPC antibody 68447-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...