Tested Applications
Positive WB detected in | HeLa cells, Jurkat cells |
Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, Jurkat cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
31175-1-AP targets HNRNPAB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HNRNPAB fusion protein Ag35010 Predict reactive species |
Full Name | heterogeneous nuclear ribonucleoprotein A/B |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 36-40 kDa |
GenBank Accession Number | BC036708 |
Gene Symbol | HNRNPAB |
Gene ID (NCBI) | 3182 |
RRID | AB_3669882 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity Purification |
UNIPROT ID | Q99729 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Heterogeneous nuclear ribonucleoprotein AB (HNRNPAB) is a member of the hnRNP family involved in multiple forms of transcriptional control. It plays an important role in normal biological processes and tumor development and progression. HNRNPAB acts as a direct transcriptional target of c-Myc and promotes G1/S cell cycle progression and cell proliferation via CDK4(PMID: 36657708).
Protocols
Product Specific Protocols | |
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WB protocol for HNRNPAB antibody 31175-1-AP | Download protocol |
IHC protocol for HNRNPAB antibody 31175-1-AP | Download protocol |
IF protocol for HNRNPAB antibody 31175-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |