HNRNPA2B1 Monoclonal antibody

• Featured Product
• KD/KO Validated

HNRNPA2B1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

3H6F7

Cat no : 67445-1-Ig

Synonyms

DKFZp779B0244, FLJ22720, hnRNP A2/B1, HNRNPA2, HNRNPA2B1, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, RNPA2, SNRPB1

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Validation Data Gallery

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  WB analysis using 67445-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 67445-1-Ig

  WB result of HNRNPA2B1 antibody (67445-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPA2B1 transfected A549 cells.

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  WB analysis using 67445-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 67445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 67445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 67445-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HNRNPA2B1 antibody (67445-1-Ig, Clone: 3H6F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of U2OS using 67445-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HNRNPA2B1 antibody (67445-1-Ig, Clone: 3H6F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human colon tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:8000-1:32000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

67445-1-Ig targets HNRNPA2B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HNRNPA2B1 fusion protein Ag6699
• Full Name: heterogeneous nuclear ribonucleoprotein A2/B1
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC000506
• Gene Symbol: HNRNPA2B1
• Gene ID (NCBI): 3181
• RRID: AB_2882679
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HnRNPA2B1 is one of heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA and participate in carcinogenesis. It has a role in packaging nascent mRNA, alternative splicing, tycoplamic RNA trafficking, translation and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport.

Publications

Species	Application	Title
human	WB,IHC	J Transl Med; HNRNPA2B1-mediated m6A modification of lncRNA MEG3 facilitates tumorigenesis and metastasis of non-small cell lung cancer by regulating miR-21-5p/PTEN axis; Authors - Ke Li ; KD Validated
human	WB	Cancer Lett; HNRNPA2B1 regulates tamoxifen- and fulvestrant-sensitivity and hallmarks of endocrine resistance in breast cancer cells.; Authors - Belinda J Petri
human	WB	Stem Cell Res Ther; Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.; Authors - Mengqi Chu ; KD Validated