HNRNPA1 Monoclonal antibody, PBS Only

HNRNPA1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A5A4

Cat no : 67844-1-PBS

Synonyms

Helix destabilizing protein, hnRNP A1, hnRNP core protein A1, HNRNPA1, HNRPA1

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Validation Data Gallery

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  WB analysis using 67844-1-Ig (same clone as 67844-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67844-1-Ig (same clone as 67844-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 67844-1-Ig (same clone as 67844-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA1 antibody (67844-1-Ig, Clone: 1A5A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67844-1-PBS targets HNRNPA1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HNRNPA1 fusion protein Ag17415
• Full Name: heterogeneous nuclear ribonucleoprotein A1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC009600
• Gene Symbol: HNRNPA1
• Gene ID (NCBI): 3178
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

In eukaryotic cells, nascent RNA polymerase II transcripts are associated in the nucleus with specific proteins to form ribonucleoprotein complexes called HNRP or 40S. Protein moiety of the HNRP particle has 6 major components called core proteins, and HNRPA1 is one of them. hnRNP A1 is a multifunctional RNA binding protein which is involved in pre-mRNA splicing, export of mature transcripts from the nucleus, mRNA turnover, and internal ribosome entry site (IRES)-mediated translation. HNRNPA1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.