HNRNPA0 Polyclonal antibody

HNRNPA0 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 10848-1-AP

Synonyms

HNRPA0, hnRNP A0

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Validation Data Gallery

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  WB analysis using 10848-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10848-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10848-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10848-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10848-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10848-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10848-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 10848-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 10848-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 10848-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IP experiment of mouse brain using 10848-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:500.

• 

  IF Staining of HeLa using 10848-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10848-1-AP (HNRNPA0 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, fetal human brain tissue, mouse brain tissue, Jurkat cells, NIH/3T3 cells
• Positive IP detected in: mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

10848-1-AP targets HNRNPA0 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HNRNPA0 fusion protein Ag1312
• Full Name: heterogeneous nuclear ribonucleoprotein A0
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC019271
• Gene Symbol: HNRNPA0
• Gene ID (NCBI): 10949
• RRID: AB_2117066
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNRNPA0 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Publications

Species	Application	Title
mouse	WB,IF	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	IF	Cell; RNA damage compartmentalization by DHX9 stress granules; Authors - Yilong Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiaochen (Verified Customer) (07-31-2023)

hnrnpa0 has many subtypes and thus got two bands, I think it is acceptable. Antibody quality is prety good.

• Applications: Western Blot

HNRNPA0 Antibody Western Blot validation ( dilution) in (Cat no:10848-1-AP)