HNMT Recombinant antibody, PBS Only

HNMT Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242159E3

Cat no : 84777-3-PBS

Synonyms

HNMT S1, HMT, Histamine N-methyltransferase, histamine N methyltransferase, EC:2.1.1.8

---

Validation Data Gallery

• 

  WB analysis using 84777-3-RR (same clone as 84777-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84777-3-RR (HNMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84777-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84777-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84777-3-RR against Human HNMT were performed. The affinity constant is below 1 pM.

---

Product Information

84777-3-PBS targets HNMT in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HNMT fusion protein Ag2462
• Full Name: histamine N-methyltransferase
• Calculated Molecular Weight: 292 aa, 33 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC020677
• Gene Symbol: HNMT
• Gene ID (NCBI): 3176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HNMT, a histamine-metabolizing enzyme, inactivates histamine by N-methylation. It plays an important role in degrading histamine and in regulating the airway response to histamine. HNMT in human astrocytes play a role in the regulation of extraneuronal histamine concentration and the activities of histaminergic neurons.