HNF4A Recombinant antibody, PBS Only

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  IF Staining of HepG2 using 84316-7-RR (same clone as 84316-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNF4A antibody (84316-7-RR, Clone: 241593A2 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84316-7-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84316-7-RR (same clone as 84316-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNF4A antibody (84316-7-RR, Clone: 241593A2 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84316-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84316-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84316-7-RR against Human HNF4A were performed. The affinity constant is 0.275 nM.

HNF4A {ptg:Brand} Recombinant Antibody for IF/ICC, Indirect ELISA

Cat No. 84316-7-PBS

Clone No.241593A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Synonyms

HNF4a8, HNF4a7, HNF4, HNF 4 alpha, Hepatocyte nuclear factor 4-alpha

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Product Information

84316-7-PBS targets HNF4A in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HNF4A fusion protein Ag24647
• Full Name: hepatocyte nuclear factor 4, alpha
• Calculated Molecular Weight: 53 kDa
• GenBank Accession Number: BC137539
• Gene Symbol: HNF4A
• Gene ID (NCBI): 3172
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P41235
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

HNF4A (Hepatocyte nuclear factor 4 α) is highly conserved between humans and mice. Using conditional knockouts, epithelial HNF4A is indispensable for protecting against acute chemical-induced colitis and spontaneous colonic inflammation in aged animals (PMID:35792863). Moreover, reduced expression of HNF4A has been reported in liver fibrosis and viral vector-mediated HNF4A restoration has previously been demonstrated to reverse features of chronic liver injury. Therefore, HNF4A has been suggested as a therapeutic target for the treatment of liver fibrosis (PMID:34453962).