HNF4A Recombinant antibody
HNF4A Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
241593C4
Cat no : 84316-3-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
84316-3-RR targets HNF4A in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | HNF4A fusion protein Ag24647 |
Full Name | hepatocyte nuclear factor 4, alpha |
Calculated Molecular Weight | 53 kDa |
Observed Molecular Weight | 56 kDa |
GenBank Accession Number | BC137539 |
Gene Symbol | HNF4A |
Gene ID (NCBI) | 3172 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HNF4A (Hepatocyte nuclear factor 4 α) is highly conserved between humans and mice. Using conditional knockouts, epithelial HNF4A is indispensable for protecting against acute chemical-induced colitis and spontaneous colonic inflammation in aged animals (PMID:35792863). Moreover, reduced expression of HNF4A has been reported in liver fibrosis and viral vector-mediated HNF4A restoration has previously been demonstrated to reverse features of chronic liver injury. Therefore, HNF4A has been suggested as a therapeutic target for treating liver fibrosis (PMID:34453962).
Protocols
Product Specific Protocols | |
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WB protocol for HNF4A antibody 84316-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |