HN1L Polyclonal antibody

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  WB analysis using 16285-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16285-1-AP (HN1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 16285-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using HN1L antibody (16285-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

HN1L Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16285-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Jupiter microtubule associated homolog 2, JPT2, HN1-like protein, Hematological and neurological expressed 1-like protein, C16orf34

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16285-1-AP targets HN1L in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HN1L fusion protein Ag9400
• Full Name: hematological and neurological expressed 1-like
• Calculated Molecular Weight: 190 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC014438
• Gene Symbol: HN1L
• Gene ID (NCBI): 90861
• RRID: AB_3085484
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H910
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hematological and neurological expressed 1-like protein (HN1L), also known as Jupiter microtubule-associated homolog 2 (JPT2), belongs to the jupiter family. HN1L is nicotinic acid adenine dinucleotide phosphate (NAADP) binding protein required for NAADP-evoked intracellular calcium release (PubMed:33758061). HN1L enables NAADP to activate Ca2+ release from the endoplasmic reticulum through ryanodine receptors (PubMed:33758062). HN1L was also required for the translocation of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus through the endolysosomal systemHN1L is expressed in liver, kidney, prostate, testis and uterus (PMID: 15094197).