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  • KD/KO Validated

HO-1/HMOX1 Polyclonal antibody

HO-1/HMOX1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF-P, CoIP, ELISA, FC (Intra)

Conjugate

Unconjugated

Cat no : 10701-1-AP

Synonyms

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HO-1, HO-1/HMOX1, HSP32



Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells, rat spleen tissue, HepG2 cells, mouse liver tissue, rat liver tissue, mouse kidney tissue, mouse spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse spleen tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10701-1-AP targets HO-1/HMOX1 in WB, IP, IHC, IF-P, CoIP, ELISA, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, chicken, monkey, pig, Bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HO-1/HMOX1 fusion protein Ag1190
Full Name heme oxygenase (decycling) 1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 28-33 kDa
GenBank Accession NumberBC001491
Gene Symbol HO-1
Gene ID (NCBI) 3162
RRIDAB_2118685
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Protocols

Product Specific Protocols
WB protocol for HO-1/HMOX1 antibody 10701-1-APDownload protocol
IHC protocol for HO-1/HMOX1 antibody 10701-1-APDownload protocol
IF protocol for HO-1/HMOX1 antibody 10701-1-APDownload protocol
IP protocol for HO-1/HMOX1 antibody 10701-1-APDownload protocol
FC protocol for HO-1/HMOX1 antibody 10701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

MFSD7c functions as a transporter of choline at the blood-brain barrier

Authors - Xuan Thi Anh Nguyen
humanWB

Nature

SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.

Authors - Ying Wang
WB

Cell Metab

Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals

Authors - Timothy C Kenny
mouseWB

Cell Metab

Functional Genomics In Vivo Reveal Metabolic Dependencies of Pancreatic Cancer Cells.

Authors - Xiphias Ge Zhu
mouseIHC

ACS Nano

Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway

Authors - Xirui Xin
humanIHC

Sci Transl Med

PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.

Authors - Tian-Yi Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Brice-Emmanuel (Verified Customer) (12-18-2023)

Works perfectly. Band a 28-33 kDa

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Mouse Heart Tissue
HO-1/HMOX1 Antibody Western Blot validation (1/1000 dilution) in Mouse Heart Tissue (Cat no:10701-1-AP)
FH

James (Verified Customer) (11-18-2022)

Very good antibody! Used it for IF staining of paraffin-embedded spleen tissue. HMOX-1 positive cells are in Green and CD45 in red.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/200
  • Cell Tissue Type: Spleen
HO-1/HMOX1 Antibody Immunofluorescence validation (1/200 dilution) in Spleen (Cat no:10701-1-AP)
FH

Hala (Verified Customer) (07-28-2021)

incubation 1.5 hours at room temperature dilution 1/3000

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/3000
  • Cell Tissue Type: HepG2
HO-1/HMOX1 Antibody Western Blot validation (1/3000 dilution) in HepG2 (Cat no:10701-1-AP)
FH

JIM (Verified Customer) (03-26-2019)

This antibody offers a good detection of HO-1 expression in LPS stimulated macrophages. The HO-1 expression can be observed in the cells after 24h stimulation of LPS.

  • Applications: Western Blot,