HO-1/HMOX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HO-1/HMOX1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

776

Reviews (3)

Cat no : 10701-1-AP

Synonyms

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HO-1, HO-1/HMOX1, HSP32

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Validation Data Gallery

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  WB analysis of HeLa using 10701-1-AP

  WB result of HMOX1 antibody (10701-1-AP, 1:3000) with si-Control and si-HMOX1 transfected HeLa cells.

• 

  WB analysis using 10701-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10701-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10701-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HMOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10701-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10701-1-AP

  IP result of anti-HO-1/HMOX1(IP:10701-1-AP, 4ug; Detection:10701-1-AP 1:20000) with HeLa cells lysate 1360 ug.

• 

  IHC staining of human liver cancer using 10701-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10701-1-AP (HMOX1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10701-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10701-1-AP (HMOX1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10701-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10701-1-AP (HMOX1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10701-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10701-1-AP (HMOX1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human liver cancer using 10701-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10701-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HO-1/HMOX1 (10701-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HT-1080 cells, HeLa cells, rat spleen tissue, HepG2 cells, mouse liver tissue, rat liver tissue, mouse kidney tissue, mouse spleen tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human liver cancer tissue
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 16 publications below
• WB: See 737 publications below
• IHC: See 62 publications below
• IF: See 54 publications below
• IP: See 2 publications below
• FC: See 2 publications below
• CoIP: See 2 publications below

Product Information

10701-1-AP targets HO-1/HMOX1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat, chicken, monkey, pig, Bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HO-1/HMOX1 fusion protein Ag1190
• Full Name: heme oxygenase (decycling) 1
• Calculated molecular weight: 33 kDa
• Observed molecular weight: 28-33 kDa
• GenBank accession number: BC001491
• Gene symbol: HMOX1
• Gene ID (NCBI): 3162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Publications

Species	Application	Title
human	WB	Nature; SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.; Authors - Ying Wang
	WB	Cell Metab; Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals; Authors - Timothy C Kenny
mouse	WB	Cell Metab; Functional Genomics In Vivo Reveal Metabolic Dependencies of Pancreatic Cancer Cells.; Authors - Xiphias Ge Zhu
human	IHC	Sci Transl Med; PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.; Authors - Tian-Yi Jiang
rat	WB	Acta Pharm Sin B; A novel PGAM5 inhibitor LFHP-1c protects blood-brain barrier integrity in ischemic stroke.; Authors - Chenglong Gao
mouse	WB	J Hazard Mater; Long-term effect of PBDE-99 prenatal exposure on spermatogenic injuries via the dysregulation of autophagy; Authors - Jun Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

James (Verified Customer) (11-18-2022)

Very good antibody! Used it for IF staining of paraffin-embedded spleen tissue. HMOX-1 positive cells are in Green and CD45 in red.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: Spleen

HO-1/HMOX1 Antibody Immunofluorescence validation (1/200 dilution) in Spleen (Cat no:10701-1-AP)

FH

Hala (Verified Customer) (07-28-2021)

incubation 1.5 hours at room temperature dilution 1/3000

• Applications: Western Blot
• Primary Antibody Dilution: 1/3000
• Cell Tissue Type: HepG2

HO-1/HMOX1 Antibody Western Blot validation (1/3000 dilution) in HepG2 (Cat no:10701-1-AP)

FH

JIM (Verified Customer) (03-26-2019)

This antibody offers a good detection of HO-1 expression in LPS stimulated macrophages. The HO-1 expression can be observed in the cells after 24h stimulation of LPS.

• Applications: Western Blot,

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