HMGN1 Polyclonal antibody

HMGN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 11695-1-AP

Synonyms

Non-histone chromosomal protein HMG-14, HMG14, High mobility group nucleosome-binding domain-containing protein 1

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Validation Data Gallery

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  WB analysis of HepG2 using 11695-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11695-1-AP (HMGN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11695-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11695-1-AP (HMGN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 11695-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11695-1-AP (HMGN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11695-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11695-1-AP (HMGN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11695-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11695-1-AP (HMGN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11695-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11695-1-AP (HMGN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11695-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HMGN1 antibody (11695-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IHC detected in: human kidney tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below

Product Information

11695-1-AP targets HMGN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HMGN1 fusion protein Ag2315
• Full Name: high-mobility group nucleosome binding domain 1
• Calculated Molecular Weight: 100 aa, 11 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC023984
• Gene Symbol: HMGN1
• Gene ID (NCBI): 3150
• RRID: AB_2248375
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HMGN1 (high-mobility group nucleosome-binding protein 1) is a member of the HMG superfamily of nonhistone chromatin-binding proteins, which which regulate chromatin structure and gene transcription. It binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. It may participate in the process which maintains transcribable genes in an unique chromatin conformation, and inhibit the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 expressed abundantly

Publications

Species	Application	Title
human	WB	J Exp Med; High-mobility group nucleosome-binding protein 1 acts as an alarmin and is critical for lipopolysaccharide-induced immune responses.; Authors - Yang De D
mouse	WB	Nat Commun; Alarmin-painted exosomes elicit persistent antitumor immunity in large established tumors in mice.; Authors - Bingfeng Zuo
human	WB	Cancer Res; The alarmin HMGN1 contributes to anti-tumor immunity and is a potent immunoadjuvant.; Authors - Feng Wei
mouse	WB	Theranostics; Alarmin augments the antitumor immunity of lentiviral vaccine in ectopic, orthotopic and autochthonous hepatocellular carcinoma mice.; Authors - Zhili Liu
human	WB	J Proteomics; Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.; Authors - Dan Zhang
mouse	IHC	PeerJ; PM2.5 exposure aggravates kidney damage by facilitating the lipid metabolism disorder in diabetic mice; Authors - Yuecheng Jiang