HMGCS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HMGCS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

39

Reviews (3)

Cat no : 17643-1-AP

Synonyms

Hydroxymethylglutaryl-CoA synthase, cytoplasmic, HMGCS, HMG-CoA synthase, HMG CoA synthase, EC:2.3.3.10

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Validation Data Gallery

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  WB analysis of HepG2 using 17643-1-AP

  WB result of HMGCS1 antibody (17643-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMGCS1 transfected HepG2 cells.

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  WB analysis using 17643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17643-1-AP (HMGCS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17643-1-AP (HMGCS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 17643-1-AP

  IP result of anti-HMGCS1 (IP:17643-1-AP, 3ug; Detection:17643-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human ovary cancer using 17643-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17643-1-AP (HMGCS1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17643-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HMGCS1 antibody (17643-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, A549 cells, HepG2 cells, LNCaP cells, mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 36 publications below
• IHC: See 7 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

17643-1-AP targets HMGCS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HMGCS1 fusion protein Ag11825
• Full Name: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)
• Calculated Molecular Weight: 520 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC083514
• Gene Symbol: HMGCS1
• Gene ID (NCBI): 3157
• RRID: AB_2248359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HMGCS1(Hydroxymethylglutaryl-CoA synthase, cytoplasmic) is also named as HMGCS and belongs to the HMG-CoA synthase family. The gene encodes a 520 amino acid protein which catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA into 3-hydroxy-3-methylglutaryl CoA and participates in the mevalonate pathway but its end product is cholesterol(PMID:20346956). The human and hamster enzymes are distinguished by differential inhibition by magnesium ion(PMID:2870496).

Publications

Species	Application	Title
human	WB	Cancer Cell; Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis; Authors - Wei Zhou ; KD Validated
human	WB	Nat Cancer; Lymphatic endothelial-like cells promote glioblastoma stem cell growth through cytokine-driven cholesterol metabolism; Authors - Linjie Zhao ; KD Validated
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
mouse,human	IF,WB	J Cell Biol; TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.; Authors - Wan Yun Ho
mouse	WB	Oxid Med Cell Longev; Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.; Authors - Wei Wang
human	WB	Mol Cell Proteomics; Quantitative proteomics reveals that enzymes of the ketogenic pathway are associated with prostate cancer progression.; Authors - Saraon Punit P

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

K (Verified Customer) (12-20-2020)

Used for western blotting (1:1000) and IHC (1:200), worked well for me

• Applications: Western Blot
• Cell Tissue Type: mouse liver tissues

FH

H (Verified Customer) (07-09-2020)

This antibody is truly good. No non-specific band detected. The target band is very strong when 15ug of cell lysate was loaded.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T and HCT116

HMGCS1 Antibody Western Blot validation (1:1000 dilution) in HEK293T and HCT116 (Cat no:17643-1-AP)

FH

Boyan (Verified Customer) (07-08-2019)

This antibody recognises a clean, and clear band at the expected molecular weight. It goes down when you treat the cells with cholesterol.

• Applications: Western Blot,
• Cell Tissue Type: MEF