HMGB1 Monoclonal antibody, PBS Only

HMGB1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D12A6

Cat no : 66525-1-PBS

Synonyms

HMG1, HMG 1, High mobility group protein B1, High mobility group protein 1, high mobility group box 1

---

Validation Data Gallery

• 

  WB analysis using 66525-1-Ig (same clone as 66525-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66525-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66525-1-Ig (same clone as 66525-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66525-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66525-1-Ig (same clone as 66525-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66525-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66525-1-Ig (same clone as 66525-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66525-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse lung using 66525-1-Ig (same clone as 66525-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using HMGB1 antibody (66525-1-Ig, Clone: 1D12A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66525-1-PBS in a different storage buffer formulation.

---

Product Information

66525-1-PBS targets HMGB1 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HMGB1 fusion protein Ag1264
• Full Name: high-mobility group box 1
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC003378
• Gene Symbol: HMGB1
• Gene ID (NCBI): 3146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).This antibody is raised against full length HMGB1 of human origin. The monomeric HMGB1 is 29 kDa and dimer is 58 kDa.