HMGA2 Polyclonal antibody

• Featured Product
• KD/KO Validated

HMGA2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

38

Reviews (3)

Cat no : 20795-1-AP

Synonyms

BABL, high mobility group AT hook 2, HMGA2, HMGI C, HMGIC, LIPO, STQTL9

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Validation Data Gallery

• 

  WB analysis using 20795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20795-1-AP (HMGA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20795-1-AP (HMGA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 20795-1-AP

  IP result of anti-HMGA2 (IP:20795-1-AP, 4ug; Detection:20795-1-AP 1:1000) with NIH/3T3 cells lysate 3200ug.

• 

  IHC staining of human pancreas cancer using 20795-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 20795-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 20795-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using HMGA2 antibody (20795-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, NIH/3T3 cells, C6 cells, HCT 116 cells, NCI-H1299 cells
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human pancreas cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 31 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below

Product Information

20795-1-AP targets HMGA2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HMGA2 fusion protein Ag14588
• Full Name: high mobility group AT-hook 2
• Calculated Molecular Weight: 108 aa, 12 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: NM_003483
• Gene Symbol: HMGA2
• Gene ID (NCBI): 8091
• RRID: AB_2665377
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HMGA2 belongs to the family of high mobility group with AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors (PMID: 18202751,19551524). HMGA2 is abundantly and ubiquitously expressed and plays a crucial role during embryonic development (18425117). HMGA2 promotes stem cell self-renewal and research studies have shown that decreased HMGA2 expression is associated with stem cell aging (19551524). Investigators have shown that expression levels of HMGA2 are very low in normal adult tissues, while either overexpression or rearrangement is associated with many types of cancer (PMID: 20228781). The calcualted molecular weight of HMGA2 is 12 kDa, but modified HMGA2 is about 18-20 kDa. (PMID: 18505920 )

Publications

Species	Application	Title
human	WB	Nat Commun; ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.; Authors - Rokana Taftaf
human	WB	Nat Commun; N6-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis.; Authors - Ri-Xin Chen
human	WB	Cancer Res; Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition; Authors - Liang Fang
mouse, rat	WB	ACS Appl Mater Interfaces; Layered Double Hydroxide Nanoparticles with Osteogenic Effects as miRNA Carriers to Synergistically Promote Osteogenesis of MSCs.; Authors - Li Yang
human	WB	Cancer Res; YTHDF1 promotes gastric carcinogenesis by controlling translation of FZD7.; Authors - Jingnan Pi
human	CoIP	J Hematol Oncol; MiR-509-3 augments the synthetic lethality of PARPi by regulating HR repair in PDX model of HGSOC.; Authors - Chenggong Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Balawant (Verified Customer) (07-25-2022)

this is very excellent antibody, I have used and it is working great. I will recumbent this antibody if you want for immunoblotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Colon tissue and renal cell line

FH

Susmita (Verified Customer) (04-13-2022)

This antibody is working fine for my WB

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 1500
• Cell Tissue Type: CRC cell line

FH

Iram (Verified Customer) (09-17-2020)

HMGA2 bands are very clear and sharp bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 for western
• Cell Tissue Type: Human colon cancer

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