HMG20B Monoclonal antibody, PBS Only

HMG20B Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C2A9

Cat no : 67354-1-PBS

Synonyms

BRAF25, BRAF35, BRCA2 associated factor 35, FLJ26127, high mobility group 20B, HMG box containing protein 20B, HMG20B, HMGX2, HMGXB2, PP7706, pp8857, SMARCE1 related protein, SMARCE1r, SOXL

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Validation Data Gallery

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  WB analysis using 67354-1-Ig (same clone as 67354-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67354-1-Ig (HMG20B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67354-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 67354-1-Ig (same clone as 67354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67354-1-Ig (HMG20B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67354-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 67354-1-Ig (same clone as 67354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67354-1-Ig (HMG20B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67354-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67354-1-PBS targets HMG20B in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HMG20B fusion protein Ag6103
• Full Name: high-mobility group 20B
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC002552
• Gene Symbol: HMG20B
• Gene ID (NCBI): 10362
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation [PMID:10449787]. The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse [PMID:15240883]. HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure [PMID:19997604].