HMG20B Monoclonal antibody

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  WB analysis using 67354-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67354-1-Ig (HMG20B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 67354-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67354-1-Ig (HMG20B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 67354-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67354-1-Ig (HMG20B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HMG20B Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67354-1-Ig

Clone No.4C2A9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

4C2A9, BRAF25, BRAF35, BRCA2 associated factor 35, BRCA2-associated factor 35

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67354-1-Ig targets HMG20B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HMG20B fusion protein Ag6103
• Full Name: high-mobility group 20B
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC002552
• Gene Symbol: HMG20B
• Gene ID (NCBI): 10362
• RRID: AB_2882609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9P0W2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation (PMID:10449787). The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse (PMID:15240883). HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure (PMID:19997604). The predicted molecular weight of the HMG20B protein is 36 kDa, while the molecular weight detected in the literature is approximately 40 kDa by SDS-PAGE (PMID: 36171271).