HMG20A Monoclonal antibody, PBS Only

HMG20A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10F12

Cat no : 66676-1-PBS

Synonyms

FLJ10739, high mobility group 20A, HMG box containing protein 20A, HMG20A, HMGX1, HMGXB1

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Validation Data Gallery

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  WB analysis of HeLa using 66676-1-Ig (same clone as 66676-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66676-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66676-1-Ig (same clone as 66676-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66676-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66676-1-Ig (same clone as 66676-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66676-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NCCIT using 66676-1-Ig (same clone as 66676-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66676-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66676-1-Ig (same clone as 66676-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66676-1-Ig (HMG20A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66676-1-PBS in a different storage buffer formulation.

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Product Information

66676-1-PBS targets HMG20A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HMG20A fusion protein Ag2718
• Full Name: high-mobility group 20A
• Calculated Molecular Weight: 347 aa, 40 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC021959
• Gene Symbol: HMG20A
• Gene ID (NCBI): 10363
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize nucleosome formation and acts as a transcription-factor-like protein that regulates the expression of several genes. HMG20A has a role in the initiation of neuronal differentiation by activating REST-responsive genes, and it also nvolves in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. HMG20A (for inhibitor of BRAF35) overcomes the repressive effects of the neuronal silencer REST and activates REST-responsive genes through the modulation of histone methylation