SPP Polyclonal antibody

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  WB analysis of HEK-293 using 20416-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20416-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 20416-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20416-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 20416-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20416-1-AP (SPP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 20416-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20416-1-AP (SPP antibody) at dilution of 1:100 (under 40x lens).

• Featured Product
• KD/KO Validated

SPP Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20416-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

H13, hIMP1, histocompatibility (minor) 13, HM13, IMP 1, IMP1, IMPAS, IMPAS 1, Intramembrane protease 1, MSTP086, Presenilin like protein 3, PSENL3, PSL3, Signal peptide peptidase, SPP

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse liver tissue, mouse kidney tissue, U2OS cells
• Positive IHC detected in: human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

20416-1-AP targets SPP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPP fusion protein Ag14158
• Full Name: histocompatibility (minor) 13
• Calculated Molecular Weight: 377 aa, 41 kDa
• Observed Molecular Weight: 45 kDa, 100 kDa
• GenBank Accession Number: BC008938
• Gene Symbol: HM13/SPP
• Gene ID (NCBI): 81502
• RRID: AB_10665421
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TCT9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Cell Sci; Topology surveillance of the lanosterol demethylase CYP51A1 by Signal Peptide Peptidase; Authors - Nikita Sergejevs ; KD Validated
		PLoS One; Visualizing active enzyme complexes using a photoreactive inhibitor for proximity ligation--application on γ-secretase.; Authors - Schedin-Weiss Sophia S
human	IHC,WB	Cell Death Dis; Histocompatibility Minor 13 (HM13), targeted by miR-760, exerts oncogenic role in breast cancer by suppressing autophagy and activating PI3K-AKT-mTOR pathway; Authors - Haiyan Yang ; KD Validated
human	WB	Front Pharmacol; Pan-cancer analysis suggests histocompatibility minor 13 is an unfavorable prognostic biomarker promoting cell proliferation, migration, and invasion in hepatocellular carcinoma; Authors - Jun Liu ; KD Validated
mouse	IHC	J Exp Clin Cancer Res; miRNA-503 inhibition exerts anticancer effects and reduces tumor growth in mesothelioma; Authors - Miriam Piccioni