HLF Polyclonal antibody, PBS Only

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  WB analysis of mouse liver using 12870-1-AP (same clone as 12870-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12870-1-AP (same clone as 12870-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 12870-1-AP (same clone as 12870-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 12870-1-AP (same clone as 12870-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 12870-1-AP (same clone as 12870-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12870-1-AP (same clone as 12870-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 12870-1-AP (same clone as 12870-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 12870-1-AP (same clone as 12870-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 12870-1-AP (same clone as 12870-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 12870-1-AP (same clone as 12870-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

HLF Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 12870-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

12870-1-PBS targets HLF in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3533

  Product name: Recombinant human HLF protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-295 aa of BC036093

  Sequence: MEKMSRPLPLNPTFIPPPYGVLRSLLENPLKLPLHHEDAFSKDKDKEKKLDDESNSPTVPQSAFLGPTLWDKTLPYDGDTFQLEYMDLEEFLSENGIPPSPSQHDHSPHPPGLQPASSAAPSVMDLSSRASAPLHPGIPSPNCMQSPIRPGQLLPANRNTPSPIDPDTIQVPVGYEPDPADLALSSIPGQEMFDPRKRKFSEEELKPQPMIKKARKVFIPDDLKDDKYWARRRKNNMAAKRSRDARRLKENQIAIRASFLEKENSALRQEVADLRKELGKCKNILAKYEARHGPL

• Full Name: hepatic leukemia factor
• Calculated Molecular Weight: 295 aa, 33 kDa
• Observed Molecular Weight: 33kDa, 66 kDa
• GenBank Accession Number: BC036093
• Gene Symbol: HLF
• Gene ID (NCBI): 3131
• RRID: AB_11182935
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16534
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Hepatic leukemia factor (Hlf), is a novel hepatic protein of the proline and acidic amino acid-rich basic leucine zipper (bZip) family, previously studied for its role in circadian rhythm regulated neurotransmitter metabolism in the brain (PMID: 29166614). HLF is an essential regulator of HSC quiescence and that HLF deficiency leads to an exhaustion of HSCs in adult mouse BM during regeneration or following cytotoxic stress (PMID: 29262330).Hlf is able to bind DNA specifically as a homodimer or as a heterodimer with other PAR factors (PMID: 1516826). Western blot analysis detected bands  33 kDa whereas the 66 kDa could be the dimer form of Hlf.