HLA-F Polyclonal antibody

HLA-F Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55379-1-AP

Synonyms

CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen, HLAF, HLA-F, Leukocyte antigen F, MHC class I antigen F

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Validation Data Gallery

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  WB analysis of Raji using 55379-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 55379-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 55379-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55379-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55379-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Raji cells, A431 cells, human placenta tissue, Jurkat cells, mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55379-1-AP targets HLA-F in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: major histocompatibility complex, class I, F
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa, 66-70 kDa
• GenBank Accession Number: NM_018950
• Gene Symbol: HLA-F
• Gene ID (NCBI): 3134
• RRID: AB_10863292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)