HLA-E Polyclonal antibody

• Featured Product
• KD/KO Validated

HLA-E Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27411-1-AP

Synonyms

EA1.2, EA2.1, HLA 6.2, HLA E, HLAE, HLA-E, MHC, MHC class I antigen E, QA1

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Validation Data Gallery

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  WB analysis of THP-1 using 27411-1-AP

  WB result of HLA-E antibody (27411-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-HLA-E transfected THP-1 cells.

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  WB analysis of human placenta using 27411-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27411-1-AP (HLA-E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27411-1-AP

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 27411-1-AP (HLA-E antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 27411-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 27411-1-AP (HLA-E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 27411-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 27411-1-AP (HLA-E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 27411-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27411-1-AP (HLA-E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27411-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27411-1-AP (HLA-E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 27411-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27411-1-AP (HLA-E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 27411-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27411-1-AP (HLA-E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue, U-937 cells, MDA-MB-231 cells, HL-60 cells, THP-1 cells
• Positive IHC detected in: human tonsillitis tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27411-1-AP targets HLA-E in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HLA-E fusion protein Ag26581
• Full Name: major histocompatibility complex, class I, E
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40-42 kDa
• GenBank Accession Number: BC002578
• Gene Symbol: HLA-E
• Gene ID (NCBI): 3133
• RRID: AB_2880865
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-E is a non-classical MHC class I molecule. HLA-E is frequently overexpressed in tumor diseases, transplants and virus-infected cells and represents an immunomodulatory molecule by binding to the receptors CD94/NKG2A, -B and -C on NK and T cells. Due to its immune suppressive features HLA-E expression might represent an important mechanism of tumors to escape immune surveillance.(PMID: 667938; 3375250; 2249951; 27589686)