HLA-E Polyclonal antibody

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• KD/KO Validated

HLA-E Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15008-1-AP

Synonyms

EA1.2, EA2.1, HLA 6.2, HLA E, HLAE, HLA-E, MHC, MHC class I antigen E, QA1

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Validation Data Gallery

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  WB analysis of THP-1 using 15008-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 15008-1-AP (HLA-E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 15008-1-AP

  WB result of HLA-E antibody (15008-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HLA-E transfected THP-1 cells.

• 

  WB analysis of human kidney using 15008-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15008-1-AP (HLA-E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15008-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15008-1-AP (HLA-E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 15008-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 15008-1-AP (HLA-E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: THP-1 cells, human kidney tissue, human liver tissue, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15008-1-AP targets HLA-E in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HLA-E fusion protein Ag6703
• Full Name: major histocompatibility complex, class I, E
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC002578
• Gene Symbol: HLA-E
• Gene ID (NCBI): 3133
• RRID: AB_1557428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-E is a non-classical MHC class I molecule. HLA-E is frequently overexpressed in tumor diseases, transplants and virus-infected cells and represents an immunomodulatory molecule by binding to the receptors CD94/NKG2A, -B and -C on NK and T cells. Due to its immune suppressive features HLA-E expression might represent an important mechanism of tumors to escape immune surveillance.(PMID: 667938; 3375250; 2249951; 27589686)

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