HLA-DRB4 Recombinant antibody, PBS Only

HLA-DRB4 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230352F10

Cat no : 83103-1-PBS

Synonyms

MHC class II antigen DRB4, HLA DRB4, HLA DR4B, 230352F10

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Validation Data Gallery

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  WB analysis using 83103-1-RR (same clone as 83103-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83103-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 83103-1-RR (same clone as 83103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83103-1-PBS in a different storage buffer formulation.

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  IF Staining of Raji using 83103-1-RR (same clone as 83103-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRB4 antibody (83103-1-RR, Clone: 230352F10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83103-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83103-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83103-1-RR against Human HLA-DRB4 were performed. The affinity constant is 0.623 nM.

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Product Information

83103-1-PBS targets HLA-DRB4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HLA-DRB4 fusion protein Ag8492
• Full Name: major histocompatibility complex, class II, DR beta 4
• Calculated Molecular Weight: 266 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC005312
• Gene Symbol: HLA-DRB4
• Gene ID (NCBI): 3126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.