HLA-DRA Monoclonal antibody, PBS Only

HLA-DRA Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D6E8

Cat no : 68543-1-PBS

Synonyms

HLA DRA, HLA DRA1, HLA-DRA, MHC class II antigen DRA

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Validation Data Gallery

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  WB analysis using 68543-1-Ig (same clone as 68543-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68543-1-Ig (HLA-DRA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68543-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Raji using 68543-1-Ig (same clone as 68543-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRA antibody (68543-1-Ig, Clone: 3D6E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68543-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68543-1-PBS targets HLA-DRA in WB, IF, Indirect ELISA applications and shows reactivity with Human, Pig samples.

• Tested Reactivity: Human, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HLA-DRA fusion protein Ag11224
• Full Name: major histocompatibility complex, class II, DR alpha
• Calculated Molecular Weight: 254 aa, 29 kDa
• Observed Molecular Weight: 32-34 kDa
• GenBank Accession Number: BC032350
• Gene Symbol: HLA-DRA
• Gene ID (NCBI): 3122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.