CoraLite®594 Anti-Human HLA-DR (L243)

HLA-DR Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

L243

Cat no : CL594-65218

Synonyms

HLA DRA, HLA DRB, HLA-DR, HLA-DRA, HLA-DRB, MHC class II antigen DR

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Validation Data Gallery

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  FC experiment of human PBMCs using CL594-65218

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human HLA-DR (CL594-65218, Clone:L243), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes and monocytes were gated.

• 

  FC experiment of human PBMCs using CL594-65218

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human HLA-DR (CL594-65218, Clone:L243), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes and monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65218 targets HLA-DR in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human lymphoblastoid B-cell line RPMI 8866
• Full Name: major histocompatibility complex, class II, DR alpha
• Calculated Molecular Weight: 254 aa, 29 kDa
• GenBank Accession Number: BC032350
• Gene Symbol: HLA-DRA
• Gene ID (NCBI): 3122
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.