Anti-Human HLA-DR (L243) Rabbit Recombinant Antibody

HLA-DR Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

L243

Cat no : 65503-1-RR

Synonyms

HLA DRA, HLA DRA1, HLA-DRA, MHC class II antigen DRA

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Validation Data Gallery

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  FC experiment of human PBMCs using 65503-1-RR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human HLA-DR Rabbit Recombinant Antibody (65503-1-RR, Clone:L243) or Rabbit IgG control PolyAb and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Lymphocytes and monocytes were gated.

• 

  FC experiment of human PBMCs using 65503-1-RR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human HLA-DR Rabbit Recombinant Antibody (65503-1-RR, Clone:L243) or Rabbit IgG control PolyAb and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Lymphocytes and monocytes were gated.

• 

  FC experiment of human PBMCs using 65503-1-RR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human HLA-DR Rabbit Recombinant Antibody (65503-1-RR, Clone:L243) (left) and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) or stained with 0.1 ug Anti-Human HLA-DR Mouse Monoclonal Antibody (Clone:L243) (center and right) and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Lymphocytes and monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65503-1-RR targets HLA-DR in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Human lymphoblastoid B-cell line RPMI 8866
• Full Name: major histocompatibility complex, class II, DR alpha
• Calculated Molecular Weight: 254 aa, 29 kDa
• GenBank Accession Number: BC032350
• Gene Symbol: HLA-DRA
• Gene ID (NCBI): 3122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

HLA-DR is a member of MHC class II moleculesencoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participate in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903). The Anti-Human HLA-DR antibody, clone L243, is derived from Human lymphoblastoid B-cell line RPMI 8866.