HLA-DQB1 Polyclonal antibody

• 

  WB analysis using 32281-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32281-1-AP (HLA-DQB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 32281-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32281-1-AP (HLA-DQB1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 32281-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32281-1-AP (HLA-DQB1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HLA-DQB1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32281-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

---

Tested Applications

• Positive WB detected in: Daudi cells, Ramos cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32281-1-AP targets HLA-DQB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HLA-DQB1 fusion protein Ag34755
• Full Name: major histocompatibility complex, class II, DQ beta 1
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC012106
• Gene Symbol: HLA-DQB1
• Gene ID (NCBI): 3119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P01920
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The HLA-DQB1 gene is part of the human leukocyte antigen (HLA) complex, which is involved in the immune system's ability to distinguish the body's own proteins from those made by foreign invaders such as viruses and bacteria. It belongs to the MHC class II genes, which provide instructions for making proteins present on the surface of certain immune system cells. These proteins attach to protein fragments (peptides) outside the cell and display them to the immune system. If the immune system recognizes the peptides as foreign, it triggers a response to attack the invading pathogens. .