HLA-DPB1 Polyclonal antibody

HLA-DPB1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11298-1-AP

Synonyms

MHC class II antigen DPB1, HLA-DP1B, HLA DPB1, HLA DP1B, DPB1

---

Validation Data Gallery

• 

  WB analysis using 11298-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 11298-1-AP (HLA-DPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 11298-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11298-1-AP (HLA-DPB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11298-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11298-1-AP (HLA-DPB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 11298-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11298-1-AP (HLA-DPB1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 11298-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11298-1-AP (HLA-DPB1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human tonsillitis using 11298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HLA-DPB1 antibody (11298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 11298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HLA-DPB1 antibody (11298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: Daudi cells
• Positive IHC detected in: human tonsillitis tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

11298-1-AP targets HLA-DPB1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HLA-DPB1 fusion protein Ag1815
• Full Name: major histocompatibility complex, class II, DP beta 1
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC015000
• Gene Symbol: HLA-DPB1
• Gene ID (NCBI): 3115
• RRID: AB_2877755
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	IHC	Sci Rep; Histological and contractile changes in the genioglossus muscle after nasal obstruction in growing rats; Authors - Karin Harumi Uchima Koecklin