HLA-DMB Polyclonal antibody

HLA-DMB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 21704-1-AP

Synonyms

RING7, MHC class II antigen DMB, HLA DMB, DMB

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Validation Data Gallery

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  WB analysis using 21704-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21704-1-AP (HLA-DMB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 21704-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21704-1-AP (HLA-DMB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 21704-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21704-1-AP (HLA-DMB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Raji using 21704-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DMB antibody (21704-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

21704-1-AP targets HLA-DMB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HLA-DMB fusion protein Ag16320
• Full Name: major histocompatibility complex, class II, DM beta
• Calculated Molecular Weight: 263 aa, 29 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC027175
• Gene Symbol: HLA-DMB
• Gene ID (NCBI): 3109
• RRID: AB_2878907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.

Publications

Species	Application	Title
human	WB	Nat Commun; Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes; Authors - Rupert L Mayer
human	WB,IF	Inflammation; RNA-seq Based Transcriptome Analysis Reveals Role of Myoglobin in Rheumatoid Arthritis; Authors - Haibin Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Adillah (Verified Customer) (04-20-2023)

good for western blot

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000