HLA-DMA Recombinant antibody, PBS Only (Detector)

HLA-DMA Recombinant Antibody for FC, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

230155D3

Cat no : 82899-1-PBS

Synonyms

D6S222E, DAMA 147C13.1 001, DAMC 226H3.5 001, DAQB 96B10.1 001, DASS 67H14.8 001, DMA, DMA protein, hCG_2001572, HLA DMA, HLA DMA protein, HLADM, HLA-DMA, RING6, XXbac BCX136A3.1 001, XXbac BPG181M17.3 001

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Validation Data Gallery

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  FC experiment of A431 using 82899-1-RR (same clone as 82899-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.13 ug Anti-Human HLA-DMA (82899-1-RR, Clone:230155D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.13 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82899-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00018-1

  Cytometric bead array standard curve ofMP00018-1, HLA-DMA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82899-5-PBS. Detection antibody: 82899-1-PBS. Standard: Ag2452. Range: 0.625-20 ng/mL.

• 

  Cytometric bead array standard curve of MP00018-3

  Cytometric bead array standard curve ofMP00018-3, HLA-DMA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82899-3-PBS. Detection antibody: 82899-1-PBS. Standard: Ag2452. Range: 0.625-20 ng/mL.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82899-1-PBS targets HLA-DMA as part of a matched antibody pair:

MP00018-1: 82899-5-PBS capture and 82899-1-PBS detection (validated in Cytometric bead array)

MP00018-3: 82899-3-PBS capture and 82899-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HLA-DMA fusion protein Ag2452
• Full Name: major histocompatibility complex, class II, DM alpha
• Calculated Molecular Weight: 261 aa, 29 kDa
• GenBank Accession Number: BC026279
• Gene Symbol: HLA-DMA
• Gene ID (NCBI): 3108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.