HLA class I (HLA-B) Polyclonal antibody

HLA class I (HLA-B) Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 17260-1-AP

Synonyms

HLA-B, MHC class I antigen, Human leukocyte antigen B, HLA class I histocompatibility antigen, B alpha chain, HLA B

---

Validation Data Gallery

• 

  WB analysis using 17260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17260-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17260-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17260-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17260-1-AP

  IP result of anti-HLA class I (HLA-B) (IP:17260-1-AP, 4ug; Detection:17260-1-AP 1:2000) with HepG2 cells lysate 4000ug.

• 

  IHC staining of human stomach cancer using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17260-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HLA class I (HLA-B) antibody (17260-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, Jurkat cells, MCF-7 cells, HeLa cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human stomach cancer tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human spleen tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

17260-1-AP targets HLA class I (HLA-B) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HLA class I (HLA-B) fusion protein Ag10947
• Full Name: major histocompatibility complex, class I, B
• Calculated Molecular Weight: 362 aa, 40 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC013187
• Gene Symbol: HLA class I (HLA-B)
• Gene ID (NCBI): 3106
• RRID: AB_1939610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-B can also react with HLA-A and HLA-C. (PMID: 667938; 3375250)

Publications

Species	Application	Title
human	IHC	Gut; Single-cell RNA sequencing reveals intrahepatic and peripheral immune characteristics related to disease phases in HBV-infected patients.; Authors - Chao Zhang
human	IF	Elife; Cell-surface tethered promiscuous biotinylators enable comparative small-scale surface proteomic analysis of human extracellular vesicles and cells.; Authors - Lisa L Kirkemo
human	WB	ACS Chem Biol; Engineering Antibodies Targeting p16 MHC-Peptide Complexes.; Authors - Nicholas J Rettko
human	WB	Cell Death Dis; Exosomal annexin A6 induces gemcitabine resistance by inhibiting ubiquitination and degradation of EGFR in triple-negative breast cancer.; Authors - Ting Li
human	WB	bioRxiv; Secreted HLA Fc-Fusion Profiles Immunopeptidome in Hypoxic PDAC and Cellular Senescence; Authors - Nicholas J Rettko
human	WB	PNAS Nexus; Secreted HLA-Fc fusion profiles immunopeptidome in hypoxic PDAC and cellular senescence; Authors - Nicholas J Rettko

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Boyan (Verified Customer) (09-16-2020)

This antibody recognises a specific band at the expected MW.

• Applications: Western Blot

Related Products

HLA class I ABC Monoclonal antibody

66013-1-Ig

HLA class I ABC Polyclonal antibody

15240-1-AP

HLA class I (HLA-C) Polyclonal antibody

15777-1-AP

Beta-2-Microglobulin Monoclonal antibody

66207-1-Ig

Beta-2-Microglobulin Polyclonal antibody

13511-1-AP

HLA-G Monoclonal antibody

66447-1-Ig

HLA-DQA1 Polyclonal antibody

16918-1-AP