HKDC1 Polyclonal antibody

HKDC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 25874-1-AP

Synonyms

hexokinase domain containing 1, HKDC1, Putative hexokinase HKDC1

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Validation Data Gallery

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  WB analysis of A549 using 25874-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25874-1-AP (HKDC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 25874-1-AP

  IP result of anti-HKDC1 (IP:25874-1-AP, 4ug; Detection:25874-1-AP 1:300) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human skeletal muscle using 25874-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25874-1-AP (HKDC1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 25874-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25874-1-AP (HKDC1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 25874-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25874-1-AP (HKDC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 25874-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25874-1-AP (HKDC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 25874-1-AP is HKDC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HKDC1 fusion protein Ag23045
• Full Name: hexokinase domain containing 1
• Calculated Molecular Weight: 917 aa, 103 kDa
• Observed Molecular Weight: 102 kDa, 82-90 kDa
• GenBank Accession Number: BC110504
• Gene Symbol: HKDC1
• Gene ID (NCBI): 80201
• RRID: AB_2880279
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Virol Sin; Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection.; Authors - Yingying Song
human	IHC,IF	Int J Mol Sci; Mitochondria-Related Transcriptome Characterization Associated with the Immune Microenvironment, Therapeutic Response and Survival Prediction in Pancreatic Cancer; Authors - Jia Dong
mouse	WB,IHC	J Mol Histol; New insights into the treatment of polycystic ovary syndrome: HKDC1 promotes the growth of ovarian granulocyte cells by regulating mitochondrial function and glycolysis; Authors - Peiwei Cong ; KD Validated
human	WB	J Cancer; HKDC1 enhances the proliferation, migration and glycolysis of pancreatic adenocarcinoma and is linked to immune infiltration; Authors - Qiang Pang