Hexokinase 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Hexokinase 1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

42

Reviews (2)

Cat no : 19662-1-AP

Synonyms

HK1, Hexokinase-A, Hexokinase-1, Hexokinase1, Hexokinase type I

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Validation Data Gallery

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  WB analysis using 19662-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19662-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 19662-1-AP

  IP result of anti-Hexokinase 1 (IP:19662-1-AP, 4ug; Detection:19662-1-AP 1:2000) with HeLa cells lysate 1720 ug.

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  IHC staining of human stomach using 19662-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 19662-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19662-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, MCF-7 cells, HepG2 cells, mouse brain tissue, rat brain tissue, THP-1 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue, human stomach tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 40 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

19662-1-AP targets Hexokinase 1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Hexokinase 1 fusion protein Ag8015
• Full Name: hexokinase 1
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC008730
• Gene Symbol: Hexokinase 1
• Gene ID (NCBI): 3098
• RRID: AB_10859778
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The hexokinases (HKs) catalyze the first obligatory step in glucose metabolism to generate glucose-6-phosphate. This not only furthers glucose entry by maintaining the concentration gradient for facilitated glucose influx but also provides the first intermediate for essentially all major pathways using glucose. There are four conventional HK isoforms, HK1/2/3/4, encoded by four different genes. Most adult tissues express only HK1. Muscle and adipose tissue use HK2 for glycolysis, liver, and pancreatic beta cells express HK4 (also called glucokinase) and do not express HK1 or HK2. (PMID: 31434645, PMID: 31848318)

Publications

Species	Application	Title
human	WB	Cell Stem Cell; Generation of human alveolar epithelial type I cells from pluripotent stem cells; Authors - Claire L Burgess
mouse	WB	Nat Immunol; NF-κB-inducing kinase maintains T cell metabolic fitness in antitumor immunity.; Authors - Meidi Gu
human,mouse	WB,IP,IF	Sci Adv; Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.; Authors - Xinwen Zhang
human	WB	Nat Commun; SUMOylation controls the binding of hexokinase 2 to mitochondria and protects against prostate cancer tumorigenesis.; Authors - Xun Shangguan
pig	WB	Br J Pharmacol; PMID: 30515768; Authors - Xiaoying Lian
human	WB	Cell Death Dis; The ubiquitin-like protein UBTD1 promotes colorectal cancer progression by stabilizing c-Myc to upregulate glycolysis; Authors - Liqin Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (01-30-2020)

Strong, clear band for mouse heart.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse Heart

FH

SCOTT (Verified Customer) (11-20-2018)

Used 1 in/1000 in 5% BSA TBS-Tween overnight at 4 C rolling. The membrane was blocked in 5% BSA TBS-Tween prior to adding the antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human induced neuronal progenitor derived astrocytes

Hexokinase 1 Antibody Western Blot, validation (1/1000 dilution) in Human induced neuronal progenitor derived astrocytes (Cat no:19662-1-AP)