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  • KD/KO Validated

Hexokinase 1 Polyclonal antibody

Hexokinase 1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 19662-1-AP


Brain form hexokinase, Cell and organelle markers, Hexokinase 1, Hexokinase type I, HK I, HK1, HK1 ta, HK1 tb, HK1 tc, HKI, HXK1

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, HepG2 cells, mouse brain tissue, rat brain tissue, THP-1 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19662-1-AP targets Hexokinase 1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Hexokinase 1 fusion protein Ag8015
Full Name hexokinase 1
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC008730
Gene Symbol HK1
Gene ID (NCBI) 3098
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The hexokinases (HKs) catalyze the first obligatory step in glucose metabolism to generate glucose-6-phosphate. This not only furthers glucose entry by maintaining the concentration gradient for facilitated glucose influx but also provides the first intermediate for essentially all major pathways using glucose. There are four conventional HK isoforms, HK1/2/3/4, encoded by four different genes. Most adult tissues express only HK1. Muscle and adipose tissue use HK2 for glycolysis, liver, and pancreatic beta cells express HK4 (also called glucokinase) and do not express HK1 or HK2. (PMID: 31434645, PMID: 31848318)


Product Specific Protocols
WB protocol for Hexokinase 1 antibody 19662-1-APDownload protocol
IHC protocol for Hexokinase 1 antibody 19662-1-APDownload protocol
IP protocol for Hexokinase 1 antibody 19662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Immunol

NF-κB-inducing kinase maintains T cell metabolic fitness in antitumor immunity.

Authors - Meidi Gu

Sci Adv

Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.

Authors - Xinwen Zhang

Nat Commun

SUMOylation controls the binding of hexokinase 2 to mitochondria and protects against prostate cancer tumorigenesis.

Authors - Xun Shangguan

Br J Pharmacol

PMID: 30515768

Authors - Xiaoying Lian

Cancer Lett

CYLD deficiency enhances metabolic reprogramming and tumor progression in nasopharyngeal carcinoma via PFKFB3.

Authors - Lingzhi Wang

PLoS Biol

Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.

Authors - Xuxiao He


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Wei (Verified Customer) (01-30-2020)

Strong, clear band for mouse heart.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Mouse Heart

SCOTT (Verified Customer) (11-20-2018)

Used 1 in/1000 in 5% BSA TBS-Tween overnight at 4 C rolling. The membrane was blocked in 5% BSA TBS-Tween prior to adding the antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human induced neuronal progenitor derived astrocytes
Hexokinase 1 Antibody Western Blot, validation (1/1000 dilution) in Human induced neuronal progenitor derived astrocytes (Cat no:19662-1-AP)