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Hexokinase 1 Polyclonal antibody
Hexokinase 1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 19662-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells, HepG2 cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 20 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
IP | See 1 publications below |
Product Information
The immunogen of 19662-1-AP is Hexokinase 1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Hexokinase 1 fusion protein Ag8015 |
Full Name | hexokinase 1 |
Calculated molecular weight | 102 kDa |
Observed molecular weight | 100 kDa |
GenBank accession number | BC008730 |
Gene symbol | HK1 |
Gene ID (NCBI) | 3098 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for Hexokinase 1 antibody 19662-1-AP | Download protocol |
IHC protocol for Hexokinase 1 antibody 19662-1-AP | Download protocol |
IP protocol for Hexokinase 1 antibody 19662-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Arthritis Res Ther Inhibition of hexokinases holds potential as treatment strategy for rheumatoid arthritis.
| ||
Anat Cell Biol Poly(ADP-ribose) polymerase regulates glycolytic activity in kidney proximal tubule epithelial cells. | ||
Reprod Fertil Dev Localisation and function of glucose transporter GLUT1 in chicken (Gallus gallus domesticus) spermatozoa: relationship between ATP production pathways and flagellar motility. | ||
Oncol Lett Downregulation of GLUT3 impairs STYK1/NOK-mediated metabolic reprogramming and proliferation in NIH-3T3 cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Wei (Verified Customer) (01-30-2020) | Strong, clear band for mouse heart.
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FH SCOTT (Verified Customer) (11-20-2018) | Used 1 in/1000 in 5% BSA TBS-Tween overnight at 4 C rolling. The membrane was blocked in 5% BSA TBS-Tween prior to adding the antibody.
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