Hexokinase 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Hexokinase 1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (2)

Cat no : 19662-1-AP

Synonyms

Brain form hexokinase, Cell and organelle markers, Hexokinase 1, Hexokinase type I, HK I, HK1, HK1 ta, HK1 tb, HK1 tc, HKI, HXK1

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Validation Data Gallery

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  WB analysis using 19662-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment using 19662-1-AP

  IP result of anti-Hexokinase 1(IP:19662-1-AP, 4ug; Detection:19662-1-AP 1:2000) with HeLa cells lysate 1720 ug.

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  IHC staining of mouse brain using 19662-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19662-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, MCF-7 cells, HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

The immunogen of 19662-1-AP is Hexokinase 1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Hexokinase 1 fusion protein Ag8015
• Full Name: hexokinase 1
• Calculated molecular weight: 102 kDa
• Observed molecular weight: 100 kDa
• GenBank accession number: BC008730
• Gene symbol: HK1
• Gene ID (NCBI): 3098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB,IHC	Arthritis Res Ther; Inhibition of hexokinases holds potential as treatment strategy for rheumatoid arthritis.; Authors - Guanhua Song ; KD Validated
pig	WB	Anat Cell Biol; Poly(ADP-ribose) polymerase regulates glycolytic activity in kidney proximal tubule epithelial cells.; Authors - Hana Song
	WB	Biomolecules; PMID: 31480692; Authors - Xixi Guo
pig	WB	Br J Pharmacol; PMID: 30515768; Authors - Xiaoying Lian
chicken	WB,IF	Reprod Fertil Dev; Localisation and function of glucose transporter GLUT1 in chicken (Gallus gallus domesticus) spermatozoa: relationship between ATP production pathways and flagellar motility.; Authors - Rangga Setiawan
mouse	WB	Oncol Lett; Downregulation of GLUT3 impairs STYK1/NOK-mediated metabolic reprogramming and proliferation in NIH-3T3 cells.; Authors - Weiye Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wei (Verified Customer) (01-30-2020)

Strong, clear band for mouse heart.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse Heart

FH

SCOTT (Verified Customer) (11-20-2018)

Used 1 in/1000 in 5% BSA TBS-Tween overnight at 4 C rolling. The membrane was blocked in 5% BSA TBS-Tween prior to adding the antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human induced neuronal progenitor derived astrocytes

Hexokinase 1 Antibody Western Blot, validation (1/1000 dilution) in Human induced neuronal progenitor derived astrocytes (Cat no:19662-1-AP)