Histone H3.4 Polyclonal antibody

Histone H3.4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18064-1-AP

Synonyms

H3.4, H3/g, H3/t, H3FT, H3t, HIST3H3, histone cluster 3, H3, Histone H3.1t, Histone H3.4

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Validation Data Gallery

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  WB analysis using 18064-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18064-1-AP (Histone H3.4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, mouse brain tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18064-1-AP targets Histone H3.4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Histone H3.4 fusion protein Ag12671
• Full Name: histone cluster 3, H3
• Calculated Molecular Weight: 136 aa, 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC101837
• Gene Symbol: Histone H3.4
• Gene ID (NCBI): 8290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIST3H3, also known as H3.1t and H3.4, is a histone H3 protein variant that bears the H3.1 variant hallmark cysteine 96 but includes three extra amino acid substitutions. H3.1t was found to be expressed in primary human spermatocytes at the pachytene stage, similar to the human testis-specific histone H1t variant, where it appears therefore to take part in the process of spermatogenesis (PMID: 21782046).