Histone H3 (C-terminal) Monoclonal antibody

Histone H3 (C-terminal) Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, chicken, zebrafish, wheat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2G10C3

Cat no : 68503-1-Ig

Synonyms

HIST1H3A, 2G10C3, Histone-H3, HistoneH3, Histone H3

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Validation Data Gallery

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  WB analysis using 68503-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68503-1-Ig (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 68503-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68503-1-Ig (HIST1H3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 68503-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68503-1-Ig (HIST1H3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, chicken brain tissue, zebrafish, wheat whole plant
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68503-1-Ig targets Histone H3 (C-terminal) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, chicken, zebrafish, wheat samples.

• Tested Reactivity: human, mouse, rat, chicken, zebrafish, wheat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.