HIRIP3 Polyclonal antibody

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  WB analysis of mouse skeletal muscle using 14992-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14992-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 14992-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14992-1-AP

  IP result of anti-HIRIP3 (IP:14992-1-AP, 4ug; Detection:14992-1-AP 1:3000) with HEK-293 cells lysate 1600 ug.

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  IHC staining of human ovary using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

HIRIP3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14992-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

HIRA-interacting protein 3

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human ovary tissue, human heart tissue, human kidney tissue, human placenta tissue, human skin tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14992-1-AP targets HIRIP3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HIRIP3 fusion protein Ag6978
• Full Name: HIRA interacting protein 3
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC000588
• Gene Symbol: HIRIP3
• Gene ID (NCBI): 8479
• RRID: AB_2117876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BW71
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. It may play a role in chromatin function and histone metabolism via its interaction with HIRA and histones.