HIRA Polyclonal antibody

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  IP experiment of HepG2 using 31152-1-AP

  IP result of anti-HIRA (IP:31152-1-AP, 4ug; Detection:31152-1-AP 1:500) with HepG2 cells lysate 1600 ug.

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  IP experiment of K-562 using 31152-1-AP

  IP result of anti-HIRA (IP:31152-1-AP, 4ug; Detection:31152-1-AP 1:500) with K-562 cells lysate 1600 ug.

HIRA Polyclonal Antibody for IP, ELISA

Cat No. 31152-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, ELISA

Synonyms

Protein HIRA, HIRA/HIR, HIR

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Tested Applications

• Positive IP detected in: HepG2 cells, K-562 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31152-1-AP targets HIRA in IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HIRA fusion protein Ag34439
• Full Name: HIR histone cell cycle regulation defective homolog A (S. cerevisiae)
• Calculated Molecular Weight: 112 kDa
• Observed Molecular Weight: 112 kDa
• GenBank Accession Number: NM_003325
• Gene Symbol: HIRA
• Gene ID (NCBI): 7290
• RRID: AB_3669873
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P54198
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIRA protein is encoded by a gene within a region of human chromosome 22q11.2 deleted in most patients with the DiGeorge syndrome, a developmental disorder, and was named for its amino acid sequence homology to two S. cerevisiae proteins, Hir1p and Hir2p. HIRA is critical in a specific chromatin assembly pathway that takes place independently of DNA synthesis (PMID: 12049744). HIRA complex associates with various proteins, such as transcription factors, RNA pol II, Prohibitin (PHB), and replication protein A (RPA) suggests that the targeting of the complex at specific locations may exploit additional structural properties and mechanisms (PMID: 30082790).