HINT3 Polyclonal antibody

HINT3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11366-1-AP

Synonyms

HINT 3, HINT3, HINT4

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Validation Data Gallery

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  WB analysis using 11366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11366-1-AP (HINT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 11366-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11366-1-AP (HINT3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 11366-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11366-1-AP (HINT3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11366-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11366-1-AP (HINT3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11366-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11366-1-AP (HINT3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells, human placenta tissue
• Positive IHC detected in: mouse liver tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11366-1-AP targets HINT3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HINT3 fusion protein Ag1912
• Full Name: histidine triad nucleotide binding protein 3
• Calculated Molecular Weight: 182 aa, 20 kDa
• Observed Molecular Weight: 20-24 kDa
• GenBank Accession Number: BC015732
• Gene Symbol: HINT3
• Gene ID (NCBI): 135114
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human Hint3 (hHint3) has been classified as a member of the histidine triad nucleotide (Hint) binding protein subfamily. HIT superfamily consists primarily of nucleoside phosphoramidases, dinucleotide hydrolases, and nucleotidyl transferases. While Hint1 is ubiquitously expressed by both eukaryotes and prokaryotes, Hint3 is found only in eukaryotes.