HINT2 Polyclonal antibody

HINT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17986-1-AP

Synonyms

HINT-3, HINT-2, HINT 3, HINT 2, EC:3.9.1.-

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Validation Data Gallery

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  WB analysis using 17986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17986-1-AP (HINT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 17986-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17986-1-AP (HINT2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17986-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HINT2 antibody (17986-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17986-1-AP targets HINT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HINT2 fusion protein Ag12413
• Full Name: histidine triad nucleotide binding protein 2
• Calculated Molecular Weight: 163 aa, 17 kDa
• Observed Molecular Weight: ~17 kDa
• GenBank Accession Number: BC047737
• Gene Symbol: HINT2
• Gene ID (NCBI): 84681
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.