Tested Applications
Positive WB detected in | HEK-293T cells, HeLa cells, rat brain tissue, rat kidney tissue, HepG2 cells |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 5 publications below |
WB | See 8 publications below |
IF | See 3 publications below |
IP | See 2 publications below |
Product Information
21749-1-AP targets HIGD1A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HIGD1A fusion protein Ag14027 Predict reactive species |
Full Name | HIG1 hypoxia inducible domain family, member 1A |
Calculated Molecular Weight | 93 aa, 10 kDa |
Observed Molecular Weight | 10 kDa |
GenBank Accession Number | BC000601 |
Gene Symbol | HIGD1A |
Gene ID (NCBI) | 25994 |
RRID | AB_10858789 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y241 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (22342701). It is induced by hypoxia induction.This antibody is a rabbit polyclonal antibody raised against a full-length human HIGD1A protein.
Protocols
Product Specific Protocols | |
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WB protocol for HIGD1A antibody 21749-1-AP | Download protocol |
IF protocol for HIGD1A antibody 21749-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Proc Natl Acad Sci U S A Higd1a is a positive regulator of cytochrome c oxidase.
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Oncogene DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.
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Mol Cell Proteomics HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV
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Mol Cell Proteomics HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.
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PLoS One Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress. |