HIF2α/EPAS1 Recombinant antibody

HIF2α/EPAS1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240736F2

Cat no : 83790-1-RR

Synonyms

EPAS1, Endothelial PAS domain-containing protein 1, ECYT4, Class E basic helix-loop-helix protein 73, bHLHe73

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Validation Data Gallery

• 

  WB analysis of A549 using 83790-1-RR

  A549 cells were subjected to SDS PAGE followed by western blot with 83790-1-RR (EPAS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 83790-1-RR

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 83790-1-RR (EPAS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83790-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83790-1-RR against Human HIF2α/EPAS1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: A549 cells, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83790-1-RR is HIF2α/EPAS1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HIF2α/EPAS1 fusion protein Ag15199
• Full Name: endothelial PAS domain protein 1
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC051338
• Gene Symbol: HIF2α
• Gene ID (NCBI): 2034
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIF2A, also named as EPAS1, is a 870 amino acid protein, which is expressed in most tissues, with highest levels in placenta, lung and heart. HIF2A colocalizes with HIF3A in the nucleus and speckles. HIF2A as a transcription factor involves in the induction of oxygen regulated genes. HIF2A binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. HIF2A regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. HIF2A may also play a role in the formation of the endothelium that gives rise to the blood brain barrier. The calculated molecular weight of HIF2A is 96 kDa, but In normoxia, HIF2A is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization. The modified HIf2A is about 100-120 kDa.