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HIF1AN Polyclonal antibody

HIF1AN Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10646-1-AP


Factor inhibiting HIF 1, FIH 1, FIH1, HIF1AN

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

10646-1-AP targets HIF1AN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIF1AN fusion protein Ag0997
Full Name hypoxia inducible factor 1, alpha subunit inhibitor
Calculated Molecular Weight 40 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC007719
Gene Symbol HIF1AN
Gene ID (NCBI) 55662
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIF1AN (Hypoxia-inducible factor 1-alpha inhibitor) is also named FIH1. It is identified as a molecular oxygen-dependent dioxygenase that blunts the transcriptional activity of HIF and has also been implicated in HIF-dependent and -independent hypoxia responses(PMID:20720525). Structural studies indicate that an FIH-1 homodimer forms in solution and is essential for FIH-1 activity(PMID:16154531).


Product Specific Protocols
WB protocol for HIF1AN antibody 10646-1-APDownload protocol
IHC protocol for HIF1AN antibody 10646-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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