HIF1AN Polyclonal antibody

• Featured Product
• KD/KO Validated

HIF1AN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10646-1-AP

Synonyms

Factor inhibiting HIF 1, FIH 1, FIH1, HIF1AN

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Validation Data Gallery

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  WB analysis of HEK-293 using 10646-1-AP

  WB result of HIF1AN antibody (10646-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIF1AN transfected HEK-293 cells.

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  WB analysis using 10646-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10646-1-AP (HIF1AN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10646-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10646-1-AP (HIF1AN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10646-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10646-1-AP (HIF1AN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

10646-1-AP targets HIF1AN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HIF1AN fusion protein Ag0997
• Full Name: hypoxia inducible factor 1, alpha subunit inhibitor
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC007719
• Gene Symbol: HIF1AN
• Gene ID (NCBI): 55662
• RRID: AB_2232963
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIF1AN (Hypoxia-inducible factor 1-alpha inhibitor) is also named FIH1. It is identified as a molecular oxygen-dependent dioxygenase that blunts the transcriptional activity of HIF and has also been implicated in HIF-dependent and -independent hypoxia responses(PMID:20720525). Structural studies indicate that an FIH-1 homodimer forms in solution and is essential for FIH-1 activity(PMID:16154531).

Publications

Species	Application	Title
rat	WB	Bioact Mater; Development of a novel RNAi therapy: Engineered miR-31 exosomes promoted the healing of diabetic wounds.; Authors - Jinghuan Huang
human	WB	Am J Cancer Res; Experimental and clinical evidence suggests that GRPEL2 plays an oncogenic role in HCC development.; Authors - Ming-Chun Lai
rat	WB	Heliyon; LncRNA OIP5-AS1 regulates ferroptosis and mitochondrial dysfunction-mediated apoptosis in spinal cord injury by targeting the miR-128-3p/Nrf2 axis; Authors - Zhensong Jiang